Analysis of the Factors that Limit the Ability of Feeder Cells to Maintain the Undifferentiated State of Human Embryonic Stem Cells

Abstract excerpt

Human embryonic stem cell (hESC) culture is routinely performed using inactivated mouse embryonic fibroblasts (MEFs) as a feeder cell layer (FL). Although these cells maintain pluripotency of hESCs, the molecular basis for this is unknown. Objectives of this study were to determine whether timing between MEF inactivation and their use as a FL influenced hESC growth and differentiation, and to begin defining the...

Topics

Share this publication in a Topic to start or enrich a Post.

• Share to 3D Printing in Biomedical Research
• Share to Pluripotent Stem Cells Research
• Share to Tissue Engineering and Regenerative Medicine