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Article

Basic Fibroblast Growth Factor Support of Human Embryonic Stem Cell Self-Renewal

10 Nov 2005

Abstract excerpt

Human embryonic stem (ES) cells have most commonly been cultured in the presence of basic fibroblast growth factor (FGF2) either on fibroblast feeder layers or in fibroblast-conditioned medium. It has recently been reported that elevated concentrations of FGF2 permit the culture of human ES cells in the absence of fibroblasts or fibroblast-conditioned medium. Herein we compare the ability of unconditioned medium...

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PMID: 16282444
DOI: 10.1634/stemcells.2005-0247

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