Article
Engineering sensitive glutathione transferase for the detection of xenobiotics.
Biosensors & bioelectronics - 15 Nov 2008
Kapoli Panagiota, Axarli Irene A, Platis Dimitris, Fragoulaki Maria, Paine Mark, Hemingway Janet, Vontas John, Labrou Nikolaos E
Abstract excerpt
Cytosolic glutathione transferases (GSTs) are a major reserve of high-capacity ligand binding proteins which recognise a large variety of hydrophobic compounds. In the present study, the binding of non-substrate xenobiotic compounds (herbicides and insecticides) to maize GST I was investigated by employing kinetic inhibition studies, site-directed mutagenesis and molecular modelling studies. The results showed...
Topics
- Binding Sites
- Biosensing Techniques
- Enzyme Stability
- Enzymes, Immobilized
- Glutathione Transferase
- Hydrogen-Ion Concentration
- Malathion
- Mutation
- Potentiometry
- Protein Engineering