Article
Chromophore motion during the bacteriorhodopsin photocycle: polarized absorption spectroscopy of bacteriorhodopsin and its M-state in bacteriorhodopsin crystals.
The EMBO journal - 1 Sept 1991
Schertler G F, Lozier R, Michel H, Oesterhelt D
Abstract excerpt
The three-dimensional crystallization of bacteriorhodopsin was systematically investigated and the needle-shaped crystal form analysed. In these crystals the M-intermediate forms 10 times faster and decays 15 times more slowly than in purple membranes. Polarized absorption spectra of the crystals...
Topics
- Bacteriorhodopsins
- Crystallization
- Halobacterium
- Kinetics
- Microspectrophotometry
- Mutation
- Photochemistry
- Protein Conformation
