Article
[Cloning cellobiohydrolase I from Penicillium decumbens 114-2 with TAIL-PCR and comparing with its derepressed mutant JU-A10].
Wei sheng wu xue bao = Acta microbiologica Sinica - 1 May 2008
Liu Ziyong, Sun Xianyun, Qu Yinbo
Abstract excerpt
OBJECTIVE: We studied the differences in gene sequence of cellobiohydrolase I gene (cbh1) from Penicillium decumbens 114-2 and its derepressed mutant JU-A10. METHODS: We cloned cbh1 and its full-length cDNA from Penicillium decumbens 114-2 by the modified thermal asymmetric interlaced polymerase chain reaction (TAIL-PCR) and RT-PCR. RESULTS: The total length of cbh1 was 1500 bp. It contained 2 introns and encoded...
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