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Article

Self-cloning baker's yeasts that accumulate proline enhance freeze tolerance in doughs.

Applied and environmental microbiology - 1 Sept 2008

Kaino Tomohiro, Tateiwa Tetsuya, Mizukami-Murata Satomi, Shima Jun, Takagi Hiroshi

Abstract excerpt

We constructed self-cloning diploid baker's yeast strains by disrupting PUT1, encoding proline oxidase, and replacing the wild-type PRO1, encoding gamma-glutamyl kinase, with a pro1(D154N) or pro1(I150T) allele. The resultant strains accumulated intracellular proline and retained higher-level fermentation abilities in the frozen doughs than the wild-type strain. These results suggest that proline-accumulating...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Source

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Identifiers

PMID: 18641164
DOI: 10.1128/AEM.00998-08
PMCID: PMC2547055

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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