Article
Selection of primers of optimal sensitivity for the detection of HIV-1 from Africa and Europe by polymerase chain reaction.
AIDS (London, England) - 1 May 1991
Grankvist O, Gustafsson A, Bredberg-Rådén U, Pallangyo K, Mhalu F, Biberfeld P, Biberfeld G, Wadell G
Abstract excerpt
In order to facilitate the detection of integrated HIV-1 proviral DNA from African as well as European patients, four new primer pairs for use in the polymerase chain reaction (PCR), localised in the gag, pol, vif and env genes of HIV-1, were constructed. The primer pairs were compared to all accessible HIV-1 sequences from African and European isolates and to some of the earlier published and most commonly used...
Topics
- Acquired Immunodeficiency Syndrome
- Africa
- Base Sequence
- DNA, Single-Stranded
- DNA, Viral
- Europe
- Genes, env
- Genes, gag
- Genes, pol
- Genes, vif
- Genetic Variation