Article
Elucidation of genetic variability among different isolates of Fasciola gigantica (giant liver fluke) using random-amplified polymorphic DNA polymerase chain reaction.
Parasitology research - 1 Oct 2008
Gunasekar K R, Tewari A K, Sreekumar C, Gupta S C, Rao J R
Abstract excerpt
The present communication focuses on molecular characterization of Fasciola gigantica isolates derived from cattle, buffalo, and goat using random-amplified polymorphic DNA (RAPD)-polymerase chain reaction (PCR) analysis to elucidate genetic variability between the three isolates. Seventeen random oligonucleotide primers of 10-11 bases with GC content varying from 50-81.8% were used in the study. Depending upon...
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