Article
Specific PCR-based assays for the identification of Fasciola species: their development, evaluation and potential usefulness in prevalence surveys.
Annals of tropical medicine and parasitology - 1 Jan 2010
Ai L, Dong S J, Zhang W Y, Elsheikha H M, Mahmmod Y S, Lin R Q, Yuan Z G, Shi Y L, Huang W Y, Zhu X Q
Abstract excerpt
Among the helminths infecting ruminants in China are three taxa belonging to the genus Fasciola: F. hepatica, F. gigantica and the so-called 'intermediate form' that appears to lie between these two species. Based on the sequences of the second internal-transcribed spacers (ITS-2) within the parasites' nuclear ribosomal DNA (rDNA), a pair of primers (DSJf/DSJ3) specific for F. hepatica and a pair (DSJf/DSJ4)...
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