Detection and quantification of <i>Mycoplasma gallisepticum</i> genome load in conjunctival samples of experimentally infected house finches ( <i>Carpodacus mexicanus</i> ) using real-time polymerase chain reaction

Abstract excerpt

A TaqMan-based real-time, quantitative polymerase chain reaction (qPCR) assay utilizing the mgc2 gene was developed to detect Mycoplasma gallisepticum in conjunctival swabs of experimentally infected house finches. The assay was demonstrated to be quantitative by the standard curve method with reproducible results within runs and between runs. The detection limit of the mgc2 assay was examined using two...