Article
Development of a duplex real-time TaqMan PCR assay with an internal control for the detection of<i>Mycoplasma gallisepticum</i>and<i>Mycoplasma synoviae</i>in clinical samples from commercial and backyard poultry
1 Apr 2010
Abstract excerpt
In this study, we report the development and validation of a duplex real-time polymerase chain reaction (PCR) assay with an internal control using TaqMan-labelled probes for the detection of Mycoplasma gallisepticum and Mycoplasma synoviae (duplex MGMS PCR). The MGMS PCR was highly specific with a sensitivity of 7 and 1 colony-forming units/ml for M. gallisepticum and M. synoviae, respectively, using dilution of...