Article
ALIS-FLP: amplified ligation selected fragment-length polymorphism method for microbial genotyping.
Scandinavian journal of clinical and laboratory investigation - 1 Jan 2008
Brillowska-Dabrowska Anna, Wianecka Magdalena, Dabrowski Sławomir, Mladenovska Zuzana, Kur Józef, Ahring Birgitte K
Abstract excerpt
A DNA fingerprinting method known as ALIS-FLP (amplified ligation selected fragment-length polymorphism) has been developed for selective and specific amplification of restriction fragments from TspRI restriction endonuclease digested genomic DNA. The method is similar to AFLP, but differs in that only one specific restriction enzyme (TspRI) is used. The cohesive ends of the DNA fragments are ligated with two...
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