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A combined AFLP-multiplex PCR assay for molecular typing of Escherichia coli strains using variable bacterial interspersed mosaic elements.

Epidemiology and infection - 1 Jan 2004

Horváth R, Dendis M, Schlegelová J, Růzicka F, Benedík J

Abstract excerpt

The original method for molecular typing of E. coli strains was developed using the polymorphism in chromosomal sequences of bacterial interspersed mosaic elements (BIMEs) detected by multiplex PCR and analysed by AFLP assay. The applicability of the method in the epidemiology of E. coli was tested on a group of 524 strains of human and veterinary origin. In the studied group 18 different genotypes were detected....

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Identifiers

PMID: 14979591
DOI: 10.1017/s095026880300133x
PMCID: PMC2870079

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