Article
Development and validation of a mismatch amplification mutation PCR assay to monitor the dissemination of an emerging variant of Vibrio cholerae O1 biotype El Tor.
Microbiology and immunology - 1 Jun 2008
Morita Masatomo, Ohnishi Makoto, Arakawa Eiji, Bhuiyan N A, Nusrin Suraia, Alam Munirul, Siddique A K, Qadri Firdausi, Izumiya Hidemasa, Nair G Balakrish, Watanabe Haruo
Abstract excerpt
A mismatch amplification mutation PCR assay was developed and validated for rapid detection of the biotype specific cholera toxin B subunit of V. cholerae O1. This assay will enable easy monitoring of the spread of a new emerging variant of the El Tor biotype of V. cholerae O1.
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