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Article

Use of automated sequencing of polymerase chain reaction-generated amplicons to identify three types of cholera toxin subunit B in Vibrio cholerae O1 strains.

Journal of clinical microbiology - 1 Jan 1993

Olsvik O, Wahlberg J, Petterson B, Uhlén M, Popovic T, Wachsmuth I K, Fields P I

Abstract excerpt

Cholera toxin is the principal factor causing the profuse intestinal fluid secretion that is characteristic of cholera. The DNA sequences of the cholera toxin subunit B structural genes from 45 Vibrio cholerae O1 strains isolated in 29 countries over a period of 70 years were determined by automated DNA sequencing of polymerase chain reaction-generated amplicons. Three types of cholera toxin B subunit gene (ctxB)...

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Identifiers

PMID: 7678018
DOI: 10.1128/jcm.31.1.22-25.1993
PMCID: PMC262614

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