Article
Novel PCR-based genotyping method, using genomic variability between repetitive sequences of toxigenic Vibrio cholerae O1 El Tor and O139.
Molecular and cellular probes - 1 Apr 2010
Tokunaga Akihiko, Yamaguchi Hiroshi, Morita Masatomo, Arakawa Eiji, Izumiya Hidemasa, Watanabe Haruo, Osawa Ro
Abstract excerpt
A novel genotyping method for toxigenic Vibrio cholerae O1 El Tor and O139 was developed. The method was designed to amplify DNA sequences "sandwiched" between any given pair of repetitive sequences, "V. cholera repeats (VCR)", in highly polymorphic "integron island" of ca. 125 kb in the small chromosome of toxigenic V. cholerae so that the resultant PCR amplicons would present with a strain-specific...
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