Article
Agrobacterium-mediated transformation of the haploid liverwort Marchantia polymorpha L., an emerging model for plant biology.
Plant & cell physiology - 1 Jul 2008
Ishizaki Kimitsune, Chiyoda Shota, Yamato Katsuyuki T, Kohchi Takayuki
Abstract excerpt
Agrobacterium-mediated transformation has not been practical in pteridophytes, bryophytes and algae to date, although it is commonly used in model plants including Arabidopsis and rice. Here we present a rapid Agrobacterium-mediated transformation system for the haploid liverwort Marchantia polymorpha L. using immature thalli developed from spores. Hundreds of hygromycin-resistant plants per sporangium were...
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