Article
A flexible loop as a functional element in the catalytic mechanism of nucleoside hydrolase from Trypanosoma vivax.
The Journal of biological chemistry - 8 Aug 2008
Vandemeulebroucke An, De Vos Stefan, Van Holsbeke Els, Steyaert Jan, Versées Wim
Abstract excerpt
The nucleoside hydrolase of Trypanosoma vivax hydrolyzes the N-glycosidic bond of purine nucleosides. Structural and kinetic studies on this enzyme have suggested a catalytic role for a flexible loop in the vicinity of the active sites. Here we present the analysis of the role of this flexible loop via the combination of a proline scan of the loop, loop deletion mutagenesis, steady state and pre-steady state...
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