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Analysis of selected transcript levels in porcine spermatozoa, oocytes, zygotes and two-cell stage embryos

10 Apr 2008

Abstract excerpt

It has been suggested that spermatozoa can deliver mRNAs to the oocyte during fertilisation. Using reverse transcription and real-time quantitative polymerase chain reaction analysis (RQ-PCR), we evaluated the presence of clusterin (CLU), protamine 2 (PRM2), calmegin (CLGN), cAMP-response element modulator protein (CREM), methyltransferase 1 (DNMT1), linker histone 1 (H1), protamine 1 (PRM1), TATA box-binding...

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PMID: 18462614
DOI: 10.1071/rd07211

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