Article
Multiple-locus variable-number tandem repeat analysis of Legionella pneumophila using multi-colored capillary electrophoresis.
Journal of microbiological methods - 1 May 2008
Nederbragt Alexander J, Balasingham Anusha, Sirevåg Reidun, Utkilen Hans, Jakobsen Kjetill S, Anderson-Glenna Mary J
Abstract excerpt
Several methods for typing of Legionella pneumophila exist, one of which is an 8-locus variable-number of tandem repeats analysis (MLVA). This method is based on separating and sizing amplified VNTR PCR products by agarose gel electrophoresis. In the present work, the existing L. pneumophila MLVA-8 assay is adapted to capillary electrophoresis. The assay was multiplexed by using multiple fluorescent labeling dyes...
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