Diversity of errantivirus (retrovirus) sequences in two cell lines used for baculovirus expression, Spodoptera frugiperda and Trichoplusia ni.

doi:10.1007/s11262-008-0221-5

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Article

Diversity of errantivirus (retrovirus) sequences in two cell lines used for baculovirus expression, Spodoptera frugiperda and Trichoplusia ni.

Virus genes - 1 Jun 2008

Menzel Tila, Rohrmann George F

Abstract excerpt

To determine the diversity of retroviruses (errantiviruses) in cell lines used for baculovirus expression, degenerate primers were designed complementary to conserved regions of lepidopteran errantivirus reverse transcriptase genes. These primers were used to PCR amplify sequences from DNA isolated from Spodoptera frugiperda (Sf-9) and Trichoplusia ni (Hi-5) cell lines. Cloning, sequencing, and phylogenetic...

Topics

Amino Acid Sequence
Animals
Baculoviridae
Cell Line
DNA, Viral
Gene Expression
Genetic Variation
Molecular Sequence Data
Moths
Phylogeny
RNA-Directed DNA Polymerase
Retroviridae
Sequence Alignment
Viral Proteins

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Identifiers

PMID: 18363091
DOI: 10.1007/s11262-008-0221-5

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