Article
Stability of a Spodoptera frugiperda nucleopolyhedrovirus deletion recombinant during serial passage in insects.
Applied and environmental microbiology - 1 Feb 2010
Simón Oihane, Williams Trevor, Possee Robert D, López-Ferber Miguel, Caballero Primitivo
Abstract excerpt
The stabilities of the Spodoptera frugiperda multiple nucleopolyhedrovirus (SfMNPV) complete genome bacmid (Sfbac) and a deletion recombinant (Sf29null) in which the Sf29 gene was replaced by a kanamycin resistance cassette were determined during sequential rounds of per os infection in insect larvae. The Sf29 gene is a viral factor that determines the number of virions in occlusion bodies (OBs). The Sf29null...
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