Article
A gene encoding a protein serine/threonine kinase is required for normal development of M. xanthus, a gram-negative bacterium.
Cell - 29 Nov 1991
Muñoz-Dorado J, Inouye S, Inouye M
Abstract excerpt
PCR reactions were carried out on the genomic DNA of M. xanthus, a soil bacterium capable of differentiation to form fruiting bodies, using oligonucleotides representing highly conserved regions of eukaryotic protein serine/threonine kinases. A gene (pkn1) thus cloned contains an ORF of 693 amino acid residues whose amino-terminal domain shows significant sequence similarity with the catalytic domain of...
Topics
- Amino Acid Sequence
- Base Sequence
- Blotting, Southern
- Chromosome Deletion
- Cloning, Molecular
- DNA, Bacterial
- Escherichia coli
- Genes, Bacterial
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Myxococcus xanthus
