Article
The tyrosine phosphatase CD148 interacts with the p85 regulatory subunit of phosphoinositide 3-kinase.
The Biochemical journal - 1 Jul 2008
Tsuboi Nobuo, Utsunomiya Tadahiko, Roberts Richard L, Ito Hideyuki, Takahashi Keiko, Noda Masaharu, Takahashi Takamune
Abstract excerpt
CD148 is a transmembrane tyrosine phosphatase that has been implicated in the regulation of cell growth and transformation. However, the signalling mechanisms of CD148 are incompletely understood. To identify the specific intracellular molecules involved in CD148 signalling, we carried out a modified yeast two-hybrid screening assay. Using the substrate-trapping mutant form of CD148 (CD148 D/A) as bait, we...
Topics
- Animals
- CHO Cells
- Cell Line
- Cricetinae
- Cricetulus
- Humans
- Mutation
- Phosphatidylinositol 3-Kinases
- Protein Binding
- Protein Subunits
- Receptor-Like Protein Tyrosine Phosphatases, Class 3
- Receptors, Platelet-Derived Growth Factor
- Two-Hybrid System Techniques