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Article

Converting human carbonic anhydrase II into a benzoate ester hydrolase through rational redesign.

Biochimica et biophysica acta - 1 May 2008

Höst Gunnar E, Jonsson Bengt-Harald

Abstract excerpt

Enzymes capable of benzoate ester hydrolysis have several potential medical and industrial applications. A variant of human carbonic anhydrase II (HCAII) was constructed, by rational design, that is capable of hydrolysing para-nitrophenyl benzoate (pNPBenzo) with an efficiency comparable to some naturally occurring esterases. The design was based on a previously developed strategy [G. Höst, L.G. Mårtensson, B.H....

Topics

Carbonic Anhydrase II
Catalysis
Esterases
Esters
Humans
Hydrolysis
Kinetics
Mutant Proteins
Mutation
Protein Engineering
Substrate Specificity

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Identifiers

PMID: 18346474
DOI: 10.1016/j.bbapap.2008.02.007

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