Article
Maturation of the yeast plasma membrane [H+]ATPase involves phosphorylation during intracellular transport.
The Journal of cell biology - 1 Oct 1991
Chang A, Slayman C W
Abstract excerpt
In this study we show that the plasma membrane [H+]ATPase of Saccharomyces cerevisiae is phosphorylated on multiple Ser and Thr residues in vivo. Phosphorylation occurs during the movement of newly synthesized ATPase from the ER to the cell surface, as revealed by the analysis of temperature-sensitive sec mutants blocked at successive steps of the secretory pathway. Two-dimensional phosphopeptide analysis of the...
Topics
- Biological Transport
- Blotting, Western
- Endoplasmic Reticulum
- Enzyme Activation
- Fungal Proteins
- Glucose
- Kinetics
- Membrane Proteins
- Mutation
- Phosphoproteins
- Phosphorylation