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Article

Agrobacterium rhizogenes-transformed roots of coffee (Coffea arabica): conditions for long-term proliferation, and morphological and molecular characterization.

Annals of botany - 1 May 2008

Alpizar E, Dechamp E, Lapeyre-Montes F, Guilhaumon C, Bertrand B, Jourdan C, Lashermes P, Etienne H

Abstract excerpt

BACKGROUND AND AIMS: The aims of this study were to set up proliferation conditions for hairy roots of Coffea arabica regenerated after transformation by Agrobacterium rhizogenes strain A4-RS, and to carry out the morphological and molecular characterization of hairy root clones maintained over the long term. METHODS: Auxin supply, light conditions and sucrose concentration were modified with the aim of...

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Identifiers

PMID: 18316320
DOI: 10.1093/aob/mcn027
PMCID: PMC2710235

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