Article
Proximal mutations at the type 1 copper site of CotA laccase: spectroscopic, redox, kinetic and structural characterization of I494A and L386A mutants.
The Biochemical journal - 1 Jun 2008
Durão Paulo, Chen Zhenjia, Silva Catarina S, Soares Cláudio M, Pereira Manuela M, Todorovic Smilja, Hildebrandt Peter, Bento Isabel, Lindley Peter F, Martins Lígia O
Abstract excerpt
In the present study the CotA laccase from Bacillus subtilis has been mutated at two hydrophobic residues in the vicinity of the type 1 copper site. The mutation of Leu(386) to an alanine residue appears to cause only very subtle alterations in the properties of the enzyme indicating minimal chan...
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