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Article

Rapid transcriptome characterization for a nonmodel organism using 454 pyrosequencing.

Molecular ecology - 1 Apr 2008

Vera J Cristobal, Wheat Christopher W, Fescemyer Howard W, Frilander Mikko J, Crawford Douglas L, Hanski Ilkka, Marden James H

Abstract excerpt

We present a de novo assembly of a eukaryote transcriptome using 454 pyrosequencing data. The Glanville fritillary butterfly (Melitaea cinxia; Lepidoptera: Nymphalidae) is a prominent species in population biology but had no previous genomic data. Sequencing runs using two normalized complementary DNA collections from a genetically diverse pool of larvae, pupae, and adults yielded 608,053 expressed sequence tags...

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PMID: 18266620
DOI: 10.1111/j.1365-294X.2008.03666.x

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

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