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Article

Population-level transcriptome sequencing of nonmodel organisms Erynnis propertius and Papilio zelicaon.

BMC genomics - 17 May 2010

O'Neil Shawn T, Dzurisin Jason D K, Carmichael Rory D, Lobo Neil F, Emrich Scott J, Hellmann Jessica J

Abstract excerpt

BACKGROUND: Several recent studies have demonstrated the use of Roche 454 sequencing technology for de novo transcriptome analysis. Low error rates and high coverage also allow for effective SNP discovery and genetic diversity estimates. However, genetically diverse datasets, such as those sourced from natural populations, pose challenges for assembly programs and subsequent analysis. Further, estimating the...

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PMID: 20478048
DOI: 10.1186/1471-2164-11-310
PMCID: PMC2887415

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