Article
Dissection of ammonium uptake systems in Corynebacterium glutamicum: mechanism of action and energetics of AmtA and AmtB.
Journal of bacteriology - 1 Apr 2008
Walter Britta, Küspert Melanie, Ansorge Daniel, Krämer Reinhard, Burkovski Andreas
Abstract excerpt
Corynebacterium glutamicum has two different Amt-type proteins. While AmtB has a low substrate affinity and is not saturable up to 3 mM methylammonium, AmtA has a high substrate affinity and mediates saturable, membrane potential-dependent transport, resulting in a high steady-state accumulation of methylammonium, even in the absence of metabolic trapping.
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