Article
Immunoblotting and ligand blotting of envelope structures of Staphylococcus aureus grown under iron insufficiency and in a sub-minimal inhibitory concentration of penicillin G.
Microbios - 1 Jan 1991
Domingue P A, Lambert P A, Brown M R
Abstract excerpt
Strip immunoblotting with specific, hyperimmune antisera and normal human sera (NHS), in conjunction with lectin and avidin ligand blotting and surface iodination, was used to investigate cell envelope components of phenotypes of Staphylococcus aureus NCTC 6571. Cocci were grown with a relatively slow doubling time (48 min) under iron sufficiency (Fe+) and iron insufficiency (Fe-), and with or without a sub-MIC...
Topics
- Animals
- Antigens, Bacterial
- Cell Membrane
- Humans
- Immune Sera
- Immunoblotting
- Iron
- Ligands
- Male
- Penicillin G
- Phenotype
- Rabbits