Article
Purification of a new restriction endonuclease from Streptococcus mutans and identification of its recognition sequence.
Acta microbiologica Hungarica - 1 Jan 1991
Molnár A, Geck P, Orosz A, Kulcsár P, Nász I
Abstract excerpt
SmuE I, a type II restriction endonuclease, has been isolated from Streptococcus mutans serotype E, which is an isoschizomes of Ava II recognizes the palidromic pentanucleotide sequence 5' GG/W/CC 3'. Similarly to Ava II, SmuE I cleaves the sequence, G--G/W/CC, generating 5' protruding fragment termini.
Topics
- Base Sequence
- Chromatography, Gel
- Culture Media
- DNA, Viral
- Deoxyribonucleases, Type II Site-Specific
- Electrophoresis, Agar Gel
- Mutation
- Plasmids
- Restriction Mapping
- Streptococcus mutans