Touchdown Enzyme Time Release-PCR for Detection and Identification of <i>Chlamydia trachomatis</i> , <i>C. pneumoniae</i> , and <i>C. psittaci</i> Using the 16S and 16S-23S Spacer rRNA Genes

Abstract excerpt

Three touchdown enzyme time release (TETR)-PCR assays were used to amplify different DNA sequences in the variable regions of the 16S and 16S-23S spacer rRNA genes specific for Chlamydia trachomatis, Chlamydia pneumoniae, and Chlamydia psittaci as improved tests for sensitive diagnosis and rapid species differentiation. The TETR-PCR protocol used 60 cycles of amplification, which provided improved analytical...

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