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Article

Genome sequencing of ion-beam-induced mutants facilitates detection of candidate genes responsible for phenotypes of mutants in rice.

Mutation research - 1 Jan 2000

Oono Yutaka, Ichida Hiroyuki, Morita Ryouhei, Nozawa Shigeki, Satoh Katsuya, Shimizu Akemi, Abe Tomoko, Kato Hiroshi, Hase Yoshihiro

Abstract excerpt

Ion beams are physical mutagens used for plant and microbe breeding that cause mutations via a mechanism distinct from those of chemical mutagens or gamma rays. We utilized whole-exome sequencing of rice DNA in order to understand the properties of ion beam-induced mutations in a genome-wide manner. DNA libraries were constructed from selected carbon-ion-beam-induced rice mutants by capturing with a custom probes...

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PMID: 32171089
DOI: 10.1016/j.mrfmmm.2020.111691

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