Article
Effects of organ culture and Optisol-GS storage on structural integrity, phenotypes, and apoptosis in cultured corneal epithelium.
Investigative ophthalmology & visual science - 1 Dec 2007
Raeder Sten, Utheim Tor P, Utheim Oygunn A, Nicolaissen Bjørn, Roald Borghild, Cai Yiqing, Haug Kristiane, Kvalheim Anders, Messelt Edvard B, Drolsum Liv, Reed John C, Lyberg Torstein
Abstract excerpt
PURPOSE: A previous report has described the use of eye bank storage of cultured human limbal epithelial cells (HLECs) to provide a reliable source of tissue for treating limbal stem cell deficiency. In the present study, conventional organ culture (OC) storage and Optisol-GS (Bausch & Lomb, Irvine, CA) storage of cultured HLECs were compared. METHODS: Three-week HLEC cultures were either organ cultured at 31...
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