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Accounting for Intra- and Intergenomic Sequence Variation in Reference Barcodes Improves eDNA Metabarcoding Biodiversity Assessment.

Molecular ecology resources - 1 Apr 2026

McCartin Luke J, Vohsen Samuel A, Wood Annemarie L, Horowitz Jeremy, Orozco-Juarbe José J, Pittoors Nicole, Morrissey Declan, Vaga Claudia F, Hansel Colleen M, Collins Allen G, Quattrini Andrea M, Herrera Santiago

Abstract excerpt

Environmental DNA (eDNA) metabarcoding can rapidly characterise biodiversity, yet its accuracy and effectiveness are limited by incomplete DNA barcode reference databases. We evaluated how comprehensive reference databases that include sequence variation within genomes (intragenomic) and across individuals and species (intergenomic) improve eDNA-based biodiversity assessments. We collected coral tissue and water...

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PMID: 41894564
DOI: 10.1111/1755-0998.70130
PMCID: PMC13028928

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