Article
Recovery of DNA for the detection and genotyping of human papillomavirus from clinical cervical specimens stored for up to 2 years in a universal collection medium with denaturing reagent.
Journal of virological methods - 1 Feb 2008
Campos Elisabete A, Simões José Antonio, Rabelo-Santos Silvia H, Sarian Luis Otávio, Pitta Denise Rocha, Levi José Eduardo, Derchain Sophie
Abstract excerpt
The recovery and stability of DNA for the detection and genotyping of HPV in UCM-containing specimens, after exposure to denaturing reagents and stored for up to 2 years were evaluated. Samples were collected from 60 women who had cervical cytology specimens harboring cervical intraepithelial neoplasia (CIN) 2 or 3. All samples were stored in UCM and had been frozen at -20 degrees C following the addition of the...
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