Article
Isolation of RNA from cell lines and cervical cytology specimens stored in BD SurePath preservative fluid and downstream detection of housekeeping gene and HPV E6 expression using real time RT-PCR.
Journal of virological methods - 1 Mar 2009
Murphy Patricia G, Henderson Dorian T, Adams Melissa D, Horlick Elizabeth A, Dixon Eric P, King Lorraine M, Avissar Patricia L, Brown Charlotte A, Fischer Timothy J, Malinowski Douglas P
Abstract excerpt
This study was performed to demonstrate that RNA isolated from cell lines and cervical cytology specimens stored in SurePath preservative fluid would be functional in real-time RT-PCR assays. RNA was isolated from cervical cell lines or cytology samples stored in SurePath preservative at room temperature for 2-5 weeks using five commercially available RNA purification kits, three of which contain proteinases. The...
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