deoP1 promoter and operator mutants in Escherichia coli: isolation and characterization.

doi:10.1111/j.1365-2958.1991.tb02083.x

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Article

deoP1 promoter and operator mutants in Escherichia coli: isolation and characterization.

Molecular microbiology - 1 Oct 1991

Dandanell G, Hammer K

Abstract excerpt

Plasmid DNA containing deoP1, one of the two major promoters of the deo operon, has been mutagenized using hydroxylamine, and promoter down-mutations and operator mutations were selected. The isolated mutants are all located within a 16 bp palindromic sequence containing the -10 region of deoP1....

Topics

Base Sequence
Cloning, Molecular
DNA, Bacterial
Escherichia coli
Hydroxylamine
Hydroxylamines
Molecular Sequence Data
Mutagenesis
Oligodeoxyribonucleotides
Operon
Phenotype
Plasmids
Promoter Regions, Genetic
Regulatory Sequences, Nucleic Acid

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