Article
Genotyping of Paracoccidioides brasiliensis directly from paraffin embedded tissue.
Medical mycology - 1 Feb 2008
Ricci Giannina, Zelck Ulrike, Mota Fernando, Lass-Flörl Cornelia, Franco Marcello F, Bialek Ralf
Abstract excerpt
Based on polymorphisms of the gp43 precursor gene, genotyping of Paracoccidioides brasiliensis was achieved in 6 out of 10 paraffin-embedded tissue samples by modifying a nested PCR procedure used in the diagnosis of the fungal infection. Nine of the samples originated in Brazil. Three sequences were identical to a previously published consensus sequence identifying the P. brasiliensis isolates as members of the...
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