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Detection of Paracoccidioides brasiliensis in Tissue Samples by a Nested PCR Assay

1 Aug 2000

Abstract excerpt

A nested PCR assay for the detection of Paracoccidioides brasiliensis DNA was evaluated, using a sequence of the immunogenic gp43 gene as a target. This gene encodes an outer membrane protein unique to this dimorphic fungus. DNA from six clinical isolates and the ATCC strain 60885 of P. brasiliensis, as well as DNA from closely related fungi, was examined to determine detection limits and cross-reactions. PCR was...

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PMID: 10921955
DOI: 10.1128/jcm.38.8.2940-2942.2000

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