Article
Genetic engineering of Pseudomonas putida KT2442 for biotransformation of aromatic compounds to chiral cis-diols.
Journal of biotechnology - 1 Nov 2007
Ouyang Shao-Ping, Liu Qian, Sun Shang-Yu, Chen Jin-Chun, Chen Guo-Qiang
Abstract excerpt
Toluene dioxygenase (TDO) catalyzes asymmetric cis-dihydroxylations of aromatic compounds. Pseudomonas putida KT2442 (pSPM01) harboring TDO genes could effectively biotransform a wide-range of aromatic substrates into their cis-diols products. In shake-flask culture, approximately 2.7gl(-1) benze...
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