Article
Genetic variability affects the detection of HIV by polymerase chain reaction.
AIDS (London, England) - 1 Aug 1991
Candotti D, Jung M, Kerouedan D, Rosenheim M, Gentilini M, M'Pele P, Huraux J M, Agut H
Abstract excerpt
Nine isolates of HIV-1 obtained from Congolese AIDS patients were amplified by the polymerase chain reaction (PCR) using primer pairs and oligomer probes derived from the HIV-1 LAV-BRU (BRU) sequence. When compared to BRU, two isolates exhibited a significant decrease of PCR efficiency with a given primer pair. Moreover, the DNA amplified from two other isolates did not hybridize with the corresponding probe...
Topics
- Base Sequence
- Congo
- DNA Probes
- DNA, Viral
- Diagnostic Errors
- Genetic Variation
- HIV Infections
- HIV-1
- Humans
- Molecular Sequence Data
- Oligodeoxyribonucleotides