Article
Destabilization of human glycine N-methyltransferase by H176N mutation.
Protein science : a publication of the Protein Society - 1 Sept 2007
Luka Zigmund, Pakhomova Svetlana, Luka Yury, Newcomer Marcia E, Wagner Conrad
Abstract excerpt
In the presence of moderate (2-4 M) urea concentrations the tetrameric enzyme, glycine N-methyltransferase (GNMT), dissociates into compact monomers. Higher concentrations of urea (7-8 M) promote complete denaturation of the enzyme. We report here that the H176N mutation in this enzyme, found in humans with hypermethioninaemia, significantly decreases stability of the tetramer, although H176 is located far from...
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