Article
Multiplex asymmetric PCR-based oligonucleotide microarray for detection of drug resistance genes containing single mutations in Enterobacteriaceae.
Antimicrobial agents and chemotherapy - 1 Oct 2007
Zhu Ling-Xiang, Zhang Zhi-Wei, Liang Dong, Jiang Di, Wang Can, Du Ning, Zhang Qiong, Mitchelson Keith, Cheng Jing
Abstract excerpt
A multiplex asymmetric PCR (MAPCR)-based microarray method was developed for the detection of 10 known extended-spectrum beta-lactamases (ESBLs) and plasmid-mediated AmpC beta-lactamase genes in gram-negative bacteria and for the typing of six important point mutations (amino acid positions 35, 43, 130, 179, 238, and 240) in the bla(SHV) gene. The MAPCR is based on a two-round reaction to promote the accumulation...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.