Article
Use of a DNA microarray for simultaneous detection of antibiotic resistance genes among staphylococcal clinical isolates.
Journal of clinical microbiology - 1 Nov 2007
Zhu Ling-Xiang, Zhang Zhi-Wei, Wang Can, Yang Hua-Wei, Jiang Di, Zhang Qiong, Mitchelson Keith, Cheng Jing
Abstract excerpt
We developed a multiplex asymmetric PCR (MAPCR)-based DNA microarray assay for characterization of the clinically relevant antibiotic resistance genes leading to penicillin, methicillin, aminoglycoside, macrolide, lincosamide, and streptogramin B (MLS(B)) resistance in staphylococci. The DNA-based assay involves detection of specific conserved regions of the mecA, blaZ (methicillin and penicillin resistance),...
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