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Reverse Line Blot Hybridization Assay for Identification of Medically Important Fungi from Culture and Clinical Specimens

19 Jul 2007

Abstract excerpt

We evaluated a combined panfungal PCR-reverse line blot (RLB) hybridization assay based on internal transcribed spacer 1 (ITS1) and ITS2 region polymorphisms to identify 159 Candida, Cryptococcus neoformans, and Aspergillus isolates (22 species). Its utility to identify fungal pathogens directly from 27 clinical specimens was also determined. ITS sequence analysis was performed to resolve discrepant...

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PMID: 17634313
DOI: 10.1128/jcm.00687-07

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