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Panfungal PCR and Multiplex Liquid Hybridization for Detection of Fungi in Tissue Specimens

1 Nov 2000

Abstract excerpt

A procedure based on panfungal PCR and multiplex liquid hybridization was developed for the detection of fungi in tissue specimens. The PCR amplified the fungal internal transcribed spacer (ITS) region (ITS1-5.8S rRNA-ITS2). After capture with specific probes, eight common fungal pathogens (Aspergillus flavus, Aspergillus fumigatus, Candida albicans, Candida krusei, Candida glabrata, Candida parapsilosis, Candida...

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PMID: 11060088
DOI: 10.1128/jcm.38.11.4186-4192.2000

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