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Article

A Novel Culture Technique for Human Embryonic Stem Cells Using Porous Membranes

12 Jul 2007

Abstract excerpt

We have developed a novel culture technique for human embryonic stem cells (hESCs) using a porous membrane with feeder cells. The feeder cells were seeded and attached to the bottom of a porous membrane and, subsequently, hESCs were cultured on the top of the membrane. This porous membrane technique (PMT) allowed hESCs to be successfully cultured and to be effectively and efficiently separated from the feeder...

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PMID: 17628020
DOI: 10.1634/stemcells.2006-0814

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